SPINEASY® PLASMID MIDIPREP KIT

Description

SPINeasy® Plasmid Midiprep Kit offers a reliable method for purification of high-copy and low-copy number
plasmid DNA from 25-50 mL of transformed bacterial culture.
The midi kit allows user to obtain high-quality plasmid DNA using spin column method without the need of
expensive accessories. Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to
release the plasmid DNA. SPINeasy® Plasmid Midiprep Kit typically produces up to 1 mg of plasmid from the
overnight culture in LB medium. High-quality plasmid DNA is immediately ready for routine molecular biology
laboratory applications.

Extraction Results

Agarose gel electrophoresis result of pcDNA 3.1 plasmid DNA extracted using SPINeasy® Plasmid Midiprep Kit.
Comparison Data of Plasmid extracted with SPINeasy® Plasmid Midiprep Kit and Competitor Kit.

Above figures show the plasmid DNA isolated according to the manufacturer’s recommended protocols from 50 mL of cultures. Each protocol was
performed in duplicate with average data shown in the table. DNA was quantified with NanoDrop.

Additional information

CAPACITY	5 preps, 50 preps