SPINEASY® HOST DEPLETION MICROBIAL DNA KIT

Description

Host DNA contamination impedes the molecular analyses of microbiomes in host samples, such as bodily
fluids and swabs.
The SPINeasy® Host Depletion Microbial DNA Kit provides an easy-to-use workflow to isolate microbial
DNA from samples containing high amounts of host DNA. This background reduction of host DNA is achieved
through selective lysis of host cells with our specially formulated Host Lysis Buffer. Microbial DNA is purified
using a convenient silica-membrane spin-column technology workflow and ready for downstream molecular
applications.

Features-

• Specially formulated saliva preservative solution to store saliva samples at room temperature for a
prolonged period
• Simple and effective extraction protocol with high DNA yield and purity
• Isolate gDNA from 25 – 500 µL of saliva with simple procedures
• Purified DNA is ready to be used for downstream application

Extraction Results-

SPINeasy Host Depletion Microbial DNA Kit demonstrates effective host DNA depletion with >90% recovery
of microbial DNA.

Gel electrophoresis of PCR amplification with DNA extracted from three saliva samples using SPINeasy Host Depletion Microbial DNA Kit. a) PCR
detection of host DNA using human β-globin primers. b) PCR detection of bacterial DNA using 16S primers.

Additional information