SPINEASY® DNA KIT FOR FECES / SOIL

Description

SPINeasy® DNA Kit for Feces/Soil is formulated to isolate high-quality DNA from both feces and soil samples
regardless of their sample complexity. Samples are optimally homogenized by bead beating method using the
new Lysing Matrix YB and lysis Buffer SF1. Subsequent treatment with Buffer SF2 effectively removes humic
acid and other contaminants. The chemistry included in Buffer SF3 enables the specific binding of DNA
without co-purification of RNA, eliminating the need for RNase A treatment.
DNA obtained from heavily contaminated soil samples showed no inhibition in PCR and was immediately ready
to be used for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing
(16S and whole genome) without the need for a further inhibitor removal step.

Features-
• Rapid and effective isolation of inhibitor free high-quality DNA from feces and soil samples in minutes
• Optimized Buffer SF3 enables the specific binding of DNA without co-purification of RNA
• New Lysing Matrix YB for effective homogenization
Extraction Results for Feces
The SPINeasy® for Feces / Soil kit provides high-quality DNA from various fecal samples.
Extraction Results for Soil
DNA Quality
Spectrophotometer

DNA yield, purity (A260/280 and A260/230 ratio) and integrity were assessed using spectrophotometer in quadruplicate and DNA gel, respectively.
Each dot of the plot represents a single extraction. The horizontal bars indicate the median value.
Amplifiability
End Point PCR Diversity

The hypervariable region V4 of the bacterial 16S rRNA gene was amplified using DNA extracted using the 4 extraction kits described in the legend.
Sequences were obtained using a NovaSeq PE250 platform and analyzed using the Qiime 2 pipeline. The relative abundance of bacterial species
compilated from 4 technical replicates is shown on the left. The percentage indicate the average proportion of gram positive firmicutes (green)
found in DNA samples derived from the same soil sample. The rarefaction curves corresponding to each method are depicted on the right. The alpha
diversity was measured by the number of operational taxonomic units (OTUs) identified (vertical axis) following the sequencing depth (horizontal axis).

Additional information