SPINEASY® DNA KIT FOR BLOOD

Description

SPINeasy® DNA Kit for Blood is a high-performance genomic DNA (gDNA) extraction kit which is based on
silica-membrane spin-column technology.
This kit enables quick isolation of gDNA from whole blood preserved in different anticoagulants (EDTA,
Heparin, and sodium citrate), typically in less than 30 minutes. It also allows easy gDNA isolation from other
samples, including plasma, serum, saliva, and cell culture medium. The use of our specially formulated Lysis
Buffer BL omits the need of homogenization and enables efficient lysis of various samples.

Features
• Rapid and efficient DNA isolation in less than 30 minutes
• Fresh, frozen, or anticoagulated blood
• Simple and effective lysis protocol using Lysis Buffer BL and Proteinase K to omit the need of any
mechanical lysis
• No organic extraction
• High purity DNA ready to be used for downstream application
Performance-
SPINeasy® DNA Kit for Blood has been tested on various sample types for its performance. The following data
demonstrate the gDNA yields obtained from various sample sources. Results showed that all extracted gDNA
are of high yield and purity (Table and Figure below) which is suitable for downstream PCR amplification.

Quality and quantity of gDNA extracted from various sample types using SPINeasy® DNA kit for Blood.
*Sample processed using homogenization method via Fastprep-24TM 5G with lysing matrix.
**Sample preserved in preservation buffer (SPS, provided in SPINeasy DNA Kit for Saliva)
Extraction Results-

M: DNA marker, DL15000
1-2: EDTA
3-4: Heparin
5-6: Sodium citrate
7-8: Blood clot
9-10: Saliva
11-12: Cell culture media
gDNA extracted from various sample using SPINeasy® DNA Kit for Blood. The purified DNA was analyzed on 1% agarose gel electrophoresis.

Additional information